Sensitive genetically encoded sensors for population and subcellular imaging of cAMP in vivo

cAMPFIRE in action. Different cAMPFIRE variants revealing cAMP dynamics in response to different norepinephrine (NE) concentrations in HEK293 cells.

Crystian I. Massengill, Landon Bayless-Edwards, Cesar C. Ceballos, Elizabeth R. Cebul, James Cahill, Arpita Bharadwaj, Evan Wilson, Maozhen Qin, Matthew R. Whorton, Isabelle Baconguis, Bing Ye, Tianyi Mao & Haining Zhong

Published in Nature Methods, the Zhong Lab and collaborators have developed a genetically encoded cAMP sensor, cleverly dubbed “cAMPFIRE”. This sensor can detect nanomolar concentrations of cAMP, can be used in vivo, and is compatible with both ratiometric and fluorescence lifetime imaging. The cAMPFIRE sensor will be a valuable tool for the scientific community, and we couldn’t be more excited about it. Check out the paper here.